Brazilein induced cells apoptosis in human lung cancer A549 cells and its effects on endoplasmic reticulum stress / 重庆医学
Chongqing Medicine
;
(36): 4180-4182, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-482118
ABSTRACT
Objective To study the apoptotic effect of brazilein on human lung cancer A549 cells and endoplasmic reticulum stress .Methods The cytotoxic activity was tested by MTT assay in A549 cells .The flow cytometry was used to detect apoptosis . Western blotting was performed to detect GRP78 and cyto c protein expression .Results The IC50 values of brazilein against A549 cells was (5 .36 ± 0 .62)μmol/L .After treatment with 0 ,5 ,10 and 20 μmol/L brazilein for 48 h ,the percent of apoptosis was (1 .15 ± 0 .32)% ,(19 .61 ± 4 .52)% ,(30 .18 ± 6 .35)% and (39 .48 ± 7 .44)% respectively .There was significant difference among the different treatment (P< 0 .05) .Compared with control group ,the protein expression of GRP78 and cytosolic cyto c was in‐creased after 5 ,10 and 20 μmol/L brazilein treated for 48 h .Conclusion Brazilein induced apoptosis in human lung cancer A549 cells though endoplasmic reticulum stress pathway .
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Chino
Revista:
Chongqing Medicine
Año:
2015
Tipo del documento:
Artículo
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