Immunohistochemical study of 14-3-3-sigma and -zeta in ovarian cancer / 부인종양
Korean Journal of Gynecologic Oncology
;
: 113-122, 2005.
Artículo
en Coreano
| WPRIM
| ID: wpr-48220
ABSTRACT
OBJECTIVE:
To evaluate the expression level, localization and distribution of 14-3-3sigma and zeta proteins in human ovarian epithelial carcinoma cells.METHODS:
Epithelial carcinoma, benign epithelial tumor and normal ovarian tissues were evaluated by immunohistochemistry. For this study, mucinous adenoma (n=10), serous adenoma (n=10), mucinous adenocarcinoma (n=10) and serous adenocarcinoma (n=10) of ovary and normal ovarian tissue (n=10) were analyzed. Goat anti 14-3-3sigma polyclonal antibody and rabbit anti-14-3-3zeta polyclonal antibody were used as primary antibodies.RESULTS:
The 14-3-3sigma protein expression in the glandular epithelium was not statistically different among normal ovary, cystadenoma and cystadenocarcinoma. The 14-3-3zeta protein expression in the glandular epithelium was significantly higher for cystadenocarcinoma than for cystadenoma and normal ovary. The 14-3-3sigma and zeta proteins were immunohistochemically demonstrated in the nuclei and more preferably in the cytoplasm of adenocarcinoma epithelial cells.CONCLUSION:
The expression of 14-3-3zeta protein increased significantly in ovarian adenocarcinoma compared with adenoma and normal ovary. It is suggested that 14-3-3zeta protein expression may play an important role in abnormal growth of ovarian epithelial tumor cell.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias Ováricas
/
Ovario
/
Cabras
/
Inmunohistoquímica
/
Adenocarcinoma
/
Adenoma
/
Adenocarcinoma Mucinoso
/
Cistadenocarcinoma
/
Cistoadenoma
/
Citoplasma
Límite:
Femenino
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Gynecologic Oncology
Año:
2005
Tipo del documento:
Artículo
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