Dexamethasone inhibits the expression of microRNA-155 in macrophages induced by lipopolysaccharide / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1591-1596, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-485600
ABSTRACT
BACKGROUND:
It is unclear about dexamethasone effect on the regulation of microRNA-155 expression in macrophages. OBJCTIVETo explore whether dexamethasone can regulate the expression of microRNA-155 in macrophages.METHODS:
(1) Lipopolysaccharide stimulation of mouse macrophages mouse macrophage cel lines, Raw264.7 cels, were culturedin vitro and stimulated by lipopolysaccharide. Cultured cels were colected at 0, 0.5, 2, 6 hours after culture to detect the dynamical expression of microRNA-155. (2) Dexamethasone intervention for macrophages Macrophages were divided into four groups control group treated with phosphate buffer; lipopolysaccharide group stimulated by lipopolysaccharide; combined group given intervention with dexamethasone and lipopolysaccharide; dexamethasone group cultured with dexamethasone. At 6 hours after culture, cel supernatant was colected to detect the expression of tumor necrosis factor α and interleukin-6 using ELISA method. Real-time fluorescence quantitative PCR was used to detect the expression of microRNA-155 in the Raw264.7 macrophages. RESULTS ANDCONCLUSION:
Lipopolysaccharide significantly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 after 6 hours of culture (P < 0.05). Combined use of dexamethasone and lipopolysaccharide slightly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 (P< 0.05). Dexamethasone alone had no influence on the expression of tumor necrosis factor α and interleukin-6, but significantly decreased the expression of microRNA-155 (P < 0.05). These findings indicate that dexamethasone can inhibit the expression of microRNA-155 in the macrophages induced by lipopolysaccharide.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2016
Tipo del documento:
Artículo
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