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Combined Treatment of Murine Fibrosarcoma with Chemotherapy (Paclitaxel), Radiotherapy, and Intratumoral Injection of Dendritic Cells
Annals of Dermatology ; : 53-60, 2014.
Artículo en Inglés | WPRIM | ID: wpr-48646
ABSTRACT

BACKGROUND:

New antitumor therapeutic strategies aim to combine different approaches that are able to induce tumor-specific effector and memory T cell responses that might control tumor growth. Dendritic cells (DCs) have the capacity to induce antigen-specific cytotoxic T lymphocytes. We have previously shown that the combined treatment of paclitaxel chemotherapy (Chemo) and injection of DCs led to complete tumor regression.

OBJECTIVE:

The goal of this study was to evaluate synergistic antitumor effect of a triple combination treatment comprising radiotherapy, paclitaxel Chemo and intratumoral injection of syngeneic bone marrow-derived DCs on murine fibrosarcoma, compared to other single or double combination treatments.

METHODS:

For the murine fibrosarcoma model, naive C57BL/6 mice were inoculated intradermally with 2x10(3) MCA102 cells in the right upper flank. Mice were assigned to five groups (untreatedcontrol, RT alone, RT+Chemo, RT+DC, and RT+Chemo+DC), with eight mice in each group. In vitro cytotoxicity assays were performed to assess the immune activity. The persistence of tumor-specific immunity was determined by second tumor challenge in mice with complete tumor regression.

RESULTS:

The triple combination treatment showed a significantly enhanced therapeutic efficacy by decreasing tumor size and inducing complete tumor regression, resulting in a cure of 50% of mice. The results of in vitro cytotoxicity assays and the second tumor challenge experiment strongly indicated the induction of a tumor-specific cytotoxic T lymphocyte response and acquisition of prolonged tumor immunity.

CONCLUSION:

These findings suggest that the triple combination treatment can be a promising strategy for the treatment of murine fibrosarcoma.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Radioterapia / Células Dendríticas / Linfocitos / Linfocitos T Citotóxicos / Paclitaxel / Terapia Combinada / Quimioterapia / Fibrosarcoma / Memoria Límite: Animales Idioma: Inglés Revista: Annals of Dermatology Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Radioterapia / Células Dendríticas / Linfocitos / Linfocitos T Citotóxicos / Paclitaxel / Terapia Combinada / Quimioterapia / Fibrosarcoma / Memoria Límite: Animales Idioma: Inglés Revista: Annals of Dermatology Año: 2014 Tipo del documento: Artículo