Effects of exogenous H2 S on O3-induced airway hyperresponsiveness and airway remodeling in a mouse model of asthma / 中国病理生理杂志

ABSTRACT

[ ABSTRACT] AIM:To investigate the effects of exogenous hydrogen sulfide ( H2 S) on ozone ( O3 )-induced air-way hyperresponsiveness and airway remodeling in a mouse model of asthma.METHODS: Female SPF C57BL/6 mice were randomized as control group, O3 group and NaHS+O3 group.Half an hour before O3 treatment, the mice in control group and O3 group were intraperitoneally injected with normal saline, while the mice in NaHS+O3 group were intraperito-neally injected with NaHS ( H2 S donor);then the mice in O3 and NaHS+O3 group were treated with O3 , while the mice in control group breathed clean air.After 4 weeks, the airway responsiveness and tracheal ring tension were measured.The bronchial basement membrane circle diameter ( Pbm) and the total area of the wall ( Wat) were determined with HE stai-ning and standardized to measure airway remodeling.The changes of goblet epithelial cells in the lung tissue were deter-mined with AB-PAS staining.RESULTS:Airway tension of O3 group was significantly higher than that in control group, but that in NaHS+O3 group was obviously lower than that in O3 group and had no significant difference compared with con-trol group.Compared with control group, the tension of tracheal ring stimulated by methacholine in O3 group increased sig-nificantly, but that in NaHS+O3 group decreased obviously compared with O3 group and had no significant change com-pared with control group.Compared with control group, bronchial wall thickness in O3 group increased significantly, but that in NaHS+O3 group decreased obviously compared with O3 group and increased significantly compared with control group.Compared with control group, goblet epithelial cell proportion in the total area of airway epithelial cells in O3 group increased significantly, but that in NaHS+O3 group decreased compared with O3 group and increased significantly com-pared with control group.CONCLUSION:Exogenous H2 S reduces O3-induced airway hyperresponsiveness and airway re-modeling, thus providing a new target for clinical drug treatment of asthma.