The effects of lactoferrin on the proliferation,migration and osteogenic differentiation of human periodontal ligament cells / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 406-411, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-490216
ABSTRACT
Objective:
To observe the effects of lactoferrin(LF)on the proliferation,migration and osteogenic differentiation of human periodontal ligament cells(HPDLCs)in vitro.Methods:
HPDLCs were cultured and identified.The proliferation and migration of HP-DLCs cultured with 0,10 and 20 μg/ml lactoferrin respectively were tested by MTT assay,Transwell assay and scratch test.The os-teogenic differentiation of the cells was evaluated using alizarin red staining and real-time PCR.Results:
Lactoferrin at 10 and 20μg/ml increased the proliferation(P <0.05),increased the quantities of mineralized nodules and the expression of alkaline phospha-tase(ALP),osteocalcin(OCN)and osteopontin(OPN)(P <0.05).Conclusion:
Lactoferrin promotes the proliferation,migration and osteogenic differentiation of HPDLCs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Practical Stomatology
Año:
2016
Tipo del documento:
Artículo
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