Migration and distribution of superparamagnetic iron oxide-labeled adipose-derived stem cells in the brain of cerebral infarction rats：MRI observation / 中国组织工程研究

ABSTRACT

BACKGROUND:To trace the survival status of transplanted stem cels and the integration with host tissues using non-invasive imaging techniques are the focus of research in recent years. OBJECTIVE:To observe the distribution and migration of superparamagnetic iron oxide (SPIO)-labeled adipose-derived stem cels in the brain of rats with cerebral infarction. METHODS:Rat models of cerebral infarction were established and randomized into SPIO-labeled group and unlabeled group. At 1 day after modeling, the rats in the two groups were given SPIO-labeled adipose-derived stem cel suspension (10 μL) and unlabeled adipose-derived stem cel suspension (10μL) into the brain, respectively. At 1, 7, 14 days after cel transplantation, neurological severity scores were measured, and MRI was used to observe the distribution of SPIO-labeled adipose-derived stem cels. RESULTS AND CONCLUSION:At 7 and 14 days after transplantation, the neurological severity scores in the two groups were significantly lower than those at 1 day after transplantation (P 0.05). At 14 days after transplantation, MRI findings showed low signals in the transplanted region, indicating the cels migrated from the corpus calosum to the lesion. These findings suggest that intracerebral transplantation of adipose-derived stem cels can promote neurological recovery from cerebral infarction in rats, and MRI can be used to visualize the distribution and migration of SPIO-labeled adipose-derived stem cels in the brain.