Calcium overload is essential for the acceleration of staurosporine-induced cell death following neuronal differentiation in PC12 cells
Experimental & Molecular Medicine
; : 269-276, 2009.
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| ID: wpr-49340
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ABSTRACT
Differentiation of neuronal cells has been shown to accelerate stress-induced cell death, but the underlying mechanisms are not completely understood. Here, we find that early and sustained increase in cytosolic ([Ca2+]c) and mitochondrial Ca2+ levels ([Ca2+]m) is essential for the increased sensitivity to staurosporine-induced cell death following neuronal differentiation in PC12 cells. Consistently, pretreatment of differentiated PC12 cells with the intracellular Ca2+-chelator EGTA-AM diminished staurosporine-induced PARP cleavage and cell death. Furthermore, Ca2+ overload and enhanced vulnerability to staurosporine in differentiated cells were prevented by Bcl-XL overexpression. Our data reveal a new regulatory role for differentiation-dependent alteration of Ca2+ signaling in cell death in response to staurosporine.
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Texto completo:
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Índice:
WPRIM
Asunto principal:
Diferenciación Celular
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Calcio
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Células PC12
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Estaurosporina
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Proteína bcl-X
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Caspasa 3
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Fragmentación del ADN
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Mitocondrias
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Neuronas
Límite:
Animals
Idioma:
En
Revista:
Experimental & Molecular Medicine
Año:
2009
Tipo del documento:
Article