HPLC Wavelength Switching Simultaneous Determination for the Contents of Mangiferin and Hesperidin inYinhua Mangguo Tablets / 中国中医药信息杂志

ABSTRACT

Objective To establish an HPLC method of wavelength switching simultaneous determination for the contents of mangiferin and hesperidin inYinhua Mangguo Tablets.Methods Agilent ZORBAX Eclipse XDB-C18 (4.6 mm×150 mm, 5 μm) column was used. The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 mL/min; the detection wavelength was 258 nm (0–20 min) for mangiferin, 283 nm (20–50 min) for hesperidin.Results Mangiferin had a good linearity in the range of 0.042–0.761 μg, and the average recovery was 99.86%, with RSD of 1.5% (n=9). Hesperidin had a good linearity in the range of 0.076–1.361 μg, and the average recovery was 99.91%, with RSD of 0.7% (n=9).Conclusion The method is simple, accurate and reproducible, which can be used to the quality control ofYinhua Mangguo Tablets.