Analysis of the changes of adhesion-related cytokines of the wound exudates after the hysteroscopy adhesion separation technique / 中国医师杂志

ABSTRACT

Objective To explore the change characteristics of platelet derived growth factor BB (PDGF-BB),transforming growth factor beta 1 (TGF-beta 1),and alkaline fibroblast factor (bFGF) of the wound exudates after the hysteroscopy adhesion separation technique.Methods Thirty two cases of intrauterine adhesions patients were selected who received the hysteroscopy adhesion separation technique from June 2014-June 2015 as the observation group,and 24 cases of hysteroscopy septum resection patients during the same period as the control group.PDGF-BB,TGF-beta 1,and bFGF of the wound exudates were measured with enzyme-linked immunosobent assay (ELISA) method at the postoperative 3,6,9,12,24,48,72 h.Results At the postoperative 3,6,9 and 12 hPDGF-BB level of the observation group was (2.73 ± 0.72) ng/ml,(3.69 ± 0.74) ng/ml,(5.78 ± 0.82) ng/ml,and (5.94 ± 0.85) ng/ml,respectively;TGF-β1 level was (3.63 ±0.57) ng/ml,(9.89 ±0.95) ng/ml,(8.24 ±0.82) ng/ml,and (7.64 ±0.75) ng/ml,respectively;bFGF level was (20.23 ± 2.35) ng/ml,(92.46 ± 23.96) ng/ml,(387.84 ±76.63)ng/ml,and (1178.53 ± 127.95)ng/ml,respectively.PDGF-BB level of the control group was (2.52 ± 0.53) ng/ml,(2.63 ± 0.55) ng/ml,(4.72 ± 0.67) ng/ml,and (4.52 ± 0.61) ng/ml,respectively;TGF-β1 level was (3.07 ±0.59)ng/ml,(7.43 ±0.67) ng/ml,(5.43 ±0.57) ng/ml,and (4.68 ±O.77)ng/ml,respectively;bFGF level was (18.25 ±2.46) ng/ml,(81.49 ±20.18) ng/ml,(237.06 ±53.97) ng/ml,and (747.02 ± 107.73) ng/ml,respectively.The PDGF-BB,TGF-beta 1,and bFGF levels of two groups at the postoperative 3,6,9 h were risen quickly,and continued to the 72 h after surgery,the peak concentration of three indices of the observation group were higher than the control group,the difference had statistical significance (P ＜ 0.05).Conclusions The levels of PDGF-BB,TGF-beta 1,and bFGF of the wound exudates were obviously increased after the hysteroscopy adhesion separation technique.