Efficient induction of gefitinib-resistant cell line HCC827 GR and its pharmacological properties / 中国药理学通报

ABSTRACT

Aim To establish a gefitinib-resistant NSCLC cell line, and observe its pharmacological properties. Methods HCC827 cells were treated with hepatocyte growth factor( HGF) and increasing concen-tration of gefitinib to induce cell resistance. MTT assay was used to test cell viability and chemosensitivity. Clone formation and Edu staining were used to verify the inhibitory effect of gefitinib on cell growth. qRT-PCR and Western blot were used to assay the expres-sion of c-Met. Results The use of HGF greatly short-ened the induction time of HCC827GR resistant cells. gefitinib could significantly suppress cell viability, col-ony formation and cell proliferation of HCC827 , but had a weaker inhibitory effect on HCC827GR cells. HCC827GR overexpressed c-Met protein, and was highly sensitive to c-Met inhibitors. Conclusion The gefitinib-resistant HCC827 GR cells were induced suc-cessfully and efficiently. The growth of HCC827GR is dependent on the activity of c-Met protein, and it can be used as a phenotypic screening model of c-Met in-hibitors.