Determination of ropivacaine in rat plasma with liquid chromatography-tandem mass spectrometry / 国际药学研究杂志

ABSTRACT

Objective To develop an LC-MS/MS method for the determination of ropivacaine in rat plasma. Methods The chromatographic separation was achieved on an Agela MP C18(2.1 mm×50 mm,3μm)analytical column. The multiple reaction monitor?ing(MRM)mode of the positive ion was adopted in the MS detection,and the precursors to the product ion transitions of m/z 275.2/126.1 and 326.0/291 were used to measure ropivacaine and internal standard(midazolam). Results The method was linear over the ropiva?caine concentration range of 0.5-250 ng/ml with the correlation coefficients r of 0.9946. The average inter-day precision values(RSD) were 1.32%-6.74%,the matrix effect values were 95.5%-102.6% and the average recovery values were 89.8%-104.7%. Conclu?sion The analysis methods for ropivacaine are simple,accurate and sensitive,thus can be used for the pharmacokinetic research of ropivacaine incorporated cubic liquid crystal in rat.