Determination of heterophyllin B inTaizishen-Huangqi oral solution by HPLC / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
;
(6): 841-843, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-498494
ABSTRACT
Objective To study a method for the determination of heterophyllin B inTaizishen- Huangqi oral solution.Methods The HPLC system consisted of Phenomenex Luna-C18 (4.6 mm × 250 mm, 5μm) column, the mobile phase consisted of acetonitrile and 0.1% acetic acid, the gradient elution flow rate was 1.0 ml/min, the column temperature was 30℃, and the UV detector was set at 203 nm.Results The linear response range was 0.016-0.320 mg/ml (r=0.999 8). The average recovery of heterophyllin B was 98.92%, and RSD was 0.80%. The average determination of Heterophyllin B within 5 batches of preparation was 1.63 mg/ml. Conclusions The method showed its high sensitivity, accurate, repeatable and high secificity. It could be used as a method to control the standard ofTaizishen-Huangqi oral solution.
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
International Journal of Traditional Chinese Medicine
Año:
2016
Tipo del documento:
Artículo
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