THE EFFECT OF AMPELOPSIN ON THE APOPTOSIS INDUCTION AND CELL GROWTH INHIBITION OF HUMAN COLON CANCER SW480 CELLS / 现代医院

ABSTRACT

Objective To investigate the effect of ampelopsin on apoptosis induction and cell growth inhibition in Human Colon cancer SW480 cells in vitro.Methods Treated with ampelopsin at several concentrations, MTT and flow cytometry was used to detect the inhibition rate and apoptotic rate of SW480 cells.Western-blot was used to investigate expression of Bcl-2 family pro-tein.Results Significant difference of cell growth inhibition rate was observed among all groups after treated with ampelopsin ( p0.05).The similar results were observed in the experiment on apoptosis induction.Level of Bcl-xL was significantly up-regulated.Level of Bax, Bid, Caspase-3, p-Ca-pase-9 and Caspase-9 was significantly down-regulated after treatment of ampelopsin.Conclusion Ampelopsin can inhibit cell growth and induce apoptosis of SW480.Bcl-2 family protein might be involved in the progress.