Quantitative analysis of γ-oryzanol content in cold pressed rice bran oil by TLC-image analysis method
Asian Pacific Journal of Tropical Biomedicine
;
(12): 119-123, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-500492
ABSTRACT
Objective:
To develop and validate an image analysis method for quantitative analysis ofγ-oryzanol in cold pressed rice bran oil.Methods:
TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. The results obtained by these two different quantification methods were compared by paired t-test.Results:
Both assays provided good linearity, accuracy, reproducibility and selectivity for determination of γ-oryzanol.Conclusions:
The TLC-densitometric and TLC-image analysis methods provided a similar reproducibility, accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil. A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods. As both methods were found to be equal, they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Asian Pacific Journal of Tropical Biomedicine
Año:
2014
Tipo del documento:
Artículo
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