Formulation Optimization of Transferrin Modified Tetrandrine and Vincristine Liposomes by Central Com-posite Design-response Surface Method / 中国药房
China Pharmacy
;
(12): 4399-4401, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-501106
ABSTRACT
OBJECTIVE:
To prepare transferrin(TF)modified tetrandrine(TET)and vincristine(VCR)active targeting lipo-somes,and to optimize its formulation.METHODS:
TF modified TET and VCR liposomes were prepared by ammonium sulfate gradient method. Using comprehensive score of encapsulation efficiency of TET and VCR as index,central composite design-re-sponse surface method was used to optimize and validate mole ratio of EPC/Chol,mole ratio of EPC/PEG2000-DSPE and TF mass fraction.RESULTS:
The optimal formulation was that the mole ratios of EPC/Chol and EPC/PEG2000-DSPE were 1.51 and 201, TF mass fraction was 0.10%. The encapsulation efficiency of TET and VCR were 97.80% and 93.00%,respectively. The compre-hensive score was 94.44(n=3)which was close to the predicted value of 93.81.CONCLUSIONS:
The optimal formulation is sta-ble and can be used for the preparation of TF modified TET and VCR liposomes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
China Pharmacy
Año:
2015
Tipo del documento:
Artículo
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