Determination of Voriconazole in Human Plasma by HPLC / 中国药房
China Pharmacy
;
(12): 4501-4503, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-501186
ABSTRACT
OBJECTIVE:
To establish a rapid,sensitive,accurate and stable method for the determination of voriconazole in human plasma in order to monitor clinical use of voriconazole.METHODS:
HPLC-UV detection method was applied using carba-mazepine as internal standard. Plasma samples were treated with acetonitrile protein precipitation. The determination was performed on Phecda C18 column with 20 mmol/L monopotassium phosphate buffer solution (pH 6.0)-acetonitrile (50∶50,V/V) as mobile phase at flow rate of 1 ml/min. The column temperature was 40℃ and detection wavelength was 255 nm. The injection volume was 50 μl.RESULTS:
The retention time of voriconazole and internal standard were 8.34 and 6.24 min. The linear range of voricon-azole in plasma were 0.10-20.00 μg/ml(r=0.999 5). The lowest limit of quantitation was 0.05 μg/ml. Intra-day and inter-day RSD were below 1.57% and 1.45%,respectively. The extraction recovery of low,medium and high concentrations were between 81.40%to 128.29%.CONCLUSIONS:
The method is simple and accurate for therapeutic drug monitoring(TDM)of voriconazole.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2015
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS