Effect of angiotensin Ⅱ on the expression of MIF in macrophages / 实用医学杂志

ABSTRACT

Objective To investigate whether angiotensin Ⅱ (AngⅡ) can regulate the expression of MIF in macrophages via the NF-κB pathway. Methods Western Blot, real time RT-PCR and ELISA were used in the present study. Results Western blot result showed that the expression of NF-κBp65 gradually increased with the increase of the concentration of AngⅡ. Results of real time RT-PCR and ELISA revealed that MIF mRNA expression and the content of MIF were significantly higher in AngⅡ group than those in the control group. PDTC could reverse the effect of AngⅡ on MIF mRNA expression and MIF secretion in macrophages. Conclusion AngⅡ can promote MIF mRNA expression and MIF secretion in macrophages via the NF-κB signaling pathway.