Effect of Aurora kinase inhibitors VX-680 on proliferation of undifferentiated thyroid carcinoma cell / 实用医学杂志

ABSTRACT

Objective To investigate the effect of Aurora-A kinase inhibitors VX-680 on the proliferation of thyroid carcinoma cell and to study their molecular mechanism. Methods The undifferentiated thyroid carcinoma cells were divided into groups of VX-680 with different drug concentration. Drug effect was observed by MTT method to measure the changes of cell proliferation. The gene expression of Aurora-A , Bcl-2 and Bax were tested by immunofluorescence assay. Results MTT results showed cell proliferation had been suppressed obviously with the of concentration increase of drug and the duration of administration (P 0.05). Conclusions Aurora-A and Bcl-2 protein expression of thyroid cancer are inhibited when VX-680 being used , at the same time , Bcl-2/Bax scale value loses balance to accelerate forming Bax/Bax homodimer. Thus cancer cell proliferation is blocked and cell apoptosis is induced. So VX-680 is a good target inhibitor for anaplasia thyroid cancer.