Simultaneous Determination of Coumarin Compounds in Yifu Pill by HPLC / 中国药房
China Pharmacy
;
(12): 4299-4301, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-503383
ABSTRACT
OBJECTIVE:
To establish a method for the simultaneous determination of coumarin compounds in Yifu pill. METH-ODSHPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-water(49∶51,V/V) at a flow rate was 1.0 ml/min,the detection wavelength was 320 nm,column temperature was 30 ℃,injection volume was 20 μl.RESULTS:
The linear range was 5.16-206.4μg/ml for osthole (r=0.999 9),37.40-1 495.89μg/ml for isoimperatorin(r=0.999 9) and 9.95-318.34 μg/ml for columbianadin(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recov-eries were 96.05%-103.19%(RSD=2.18%,n=9) for osthole,96.90%-103.09%(RSD=2.07%,n=9) for isoimperatorin, 95.50%-103.57%(RSD=2.15%,n=9)for columbianadin.CONCLUSIONS:
The method is simple with good precision,stability and reproducibility,and can be used for the simultaneous determination of coumarin compounds in Yifu pill.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2016
Tipo del documento:
Artículo
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