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Effects of Propofol on Hepatoma Carcinoma HepG2 Cell Invasion / 中国药房
China Pharmacy ; (12): 899-902, 2016.
Article en Zh | WPRIM | ID: wpr-504327
Biblioteca responsable: WPRO
ABSTRACT
OBJECTIVE:To study the effects of propofol on invasion of hepatoma carcinoma HepG2 cell. METHODS:MTT method was used to detect the viability of HepG2 cells which were cultured with 0(negative control),1,3 and 10 μg/ml propofol for 48 h. The ability of cell invasion was detect by Tranaswell method. The phosphorylation level of nuclear factor-κB p65(NF-κB p65) and the expression of matrix metalloproteinase 2 (MMP-2),MMP-9,E-cadhherin and Snail were detected by Western blot method. RESULTS:Compared with negative control,1,3,10 μg/ml propofol inhibited the cell viability and invasion,down-regu-lated the expression of Snail and the phosphorylation level of NF-κB p65,and up-regulated the expression of E-cadherin (P<0.01). 3,10μg/ml propofol down-regulated the expression of MMP-2 and MMP-9(P<0.01). Above effects depended on drug con-centration(P<0.01). CONCLUSIONS:Propofol can suppress HepG2 invasion,which might be related to the inhibition of NF-κB/Snail signal pathway.
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Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: China Pharmacy Año: 2016 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: China Pharmacy Año: 2016 Tipo del documento: Article