Enforced expression Foxp3 enhances proliferation and tumorigeneity of lung cancer cell / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1481-1484, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-504353
ABSTRACT
Objective:
To determine the effects of enforced expression of Foxp3 in lung cancer cell with regards to proliferation and tumorgeneity.Methods:
A stable subline NCIH-hFoxp3 was established by liopfectamin-mediated pcDNA plasmid transfection carrying exogenous hFoxp3. The growth curve and secrection of IL-8 and IL-10 of NCIH-hFoxp3 were evaluated using MTT and ELISA, respectively. The in vivo tumorigeneity was assessed as well by inoculation of NCIH-hFoxp3 subcutaneously in nude mice.Results:
Lung cancer cell NCIH-hFoxp3 with enforced expression of Foxp3 proliferated slowly but exihited increased in vivo tumorgeneity compared with corresponding control subline. In addition,increased expression of hFoxp3 in NCIH-hFoxp3 augmented secretion and at-tenuated secretion of IL-8 and IL-10,respectively.Conclusion:
Increased expression of Foxp3 may promote progression of lung cancer cell by change of cellular microenvironment and evasion of immune surveillance.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Immunology
Año:
2016
Tipo del documento:
Artículo
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