Determination of Rosmarinic Acid in Herba Rabdosiae Serrae by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for rosmarinic acid in Herba Rabdosiae Serrae. METHODS:HPLC method was performed on the column of Inertsil ODS-SP C18 with mobile phase of acetonitrile-0.4% phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 327 nm,column temperature was 25 ℃,and injection volume 10 μl. RE-SULTS:The linear range of rosmarinic acid was 5.039-100.776 μg/ml(r=0.999 6),RSDs of precision,stability and reproducibility tests were lower than 3%;recovery was 99.53-104.38%(RSD=2.06%,n=9). CONCLUSIONS:The method is simple with good reproducibility and high accuracy,and can be used for the content determination of rosmarinic acid in Herba Rabdosiae Serrae.