Study on Isolation and Structure Characteristics of Immunoactive Polysaccharide RTPS-Ⅰfrom Trichosan-this Radix / 中国药师
China Pharmacist
;
(12): 270-273, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-507503
ABSTRACT
Objective:
To separate and purify the immunoactive polysaccharide RTPS- I from Trichosanthis Radix and study the physicochemical properties and preliminary structure.Methods:
The crude polysaccharides were extracted by water, and then precipita-ted by ethanol. After the deproteinization with trichloroacetic acid, the polysaccharide RTPS-I was separated and purified by using DE-AE-Cellulose, DEAE-Sepharose FF and Sephadex G-200 column chromatography. The analysis of properties and structure was per-formed by using GC, HPGPC, IR and NMR.Results:
RTPS-I was a white flocculent solid after vacuum freeze-drying, and soluble in water. It contained three elements (carbon, hydrogen and oxygen) with the specific rotation [α]25D (H2O) of +164. 44 and the relative molecular mass of 17555. The glycosyl in RTPS- I was composed of glucose without uronic acid. The sugar ring was pyranoid ring, and each monosaccharide was linked by α-glycosidic bond.Conclusion:
A homogeneous immunoactive polysaccharide RTPS- I from Trichosanthis Radix is isolated for the first time.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacist
Año:
2017
Tipo del documento:
Artículo
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