Determination of 4-Hydrazinobenzenesulfonamide Hydrochloride in Celecoxib by HPLC / 中国药师
China Pharmacist
; (12): 360-362, 2017.
Article
en Zh
| WPRIM
| ID: wpr-507554
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WPRO
ABSTRACT
Objective:To establish a method for the determination of 4-hydrazinobenzenesulfonamide hydrochloride in celecoxib. Methods:HPLC was used and the chromatographic column was a Hypercarb series C18 column (150 mm × 4. 6 mm, 3 μm) with 100% porous graphite as the filler. The mobile phase consisted of 10 mmol·L-1 ammonium acetate aqueous solution-methanol (50 :50) and methanol. The flow rate was 0. 8 ml·min-1 and the detection wavelength was 220 nm. The column temperature was 35 ℃and the injection volume was 20 μl. Results:The linear range was 99. 897-599. 382 ng·ml-1(r=0. 9982) for 4-hydrazinobenzene-sulfonamide hydrochloride. The average recovery was 97. 4%(RSD=3. 0%,n=9). Conclusion:The method is simple and accurate, and can be used for the determination of 4-hydrazinobenzenesulfonamide hydrochloride in celecoxib.
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Zh
Revista:
China Pharmacist
Año:
2017
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Article