Breeding of Zmu-1:DHP inbred strain guinea pig and preliminary analysis of molecular genetic structure of the strain / 中国实验动物学报

ABSTRACT

Objective To breed a guinea pig inbred strain and set up a method for detection of the microsatellite markers of genetic structure in guinea pigs. Method Using inbreeding methods we try to breed the Zmu-1DHP inbred strain. With 15 pairs of polymorphism microsatellite primers, the genetic homozygosity of Zmu?1DHP inbred strain,Zmu?1DHP outbred strain and Zmu?2DHP inbred strain ( as control) were examined by PCR. Results After breeding for 13 years, 8 sublines of Zmu?1DHP inbred strain ( >20 generations) were bred. After identification, the gene frequency of the second subline of Zmu?1DHP inbred strain was 86. 7%,higher than Zmu?1DHP outbred strain (6. 7%) and Zmu?2DHP inbred strain (66. 7%). The average number of loci of Zmu?1DHP inbred strain was 1. 13,lower than that of Zmu?1DHP outbred strain (2. 47%) and Zmu?2DHP inbred strain (1. 33%). The genotypic frequency of Zmu?1DHP in?bred strain was also higher than that of the other strains. The gene types of Zmu?1DHP inbred strain were included in the genes of Zmu?1DHP outbred strain, but Zmu?1DHP inbred strain was short of 2 characteristic genes. The gene homozy?gous rates of 8 sublines of Zmu?1DHP inbred strain were different with each other,among them, those of the 2nd and 8th sublines were higher than others. Conclusions There are both homozygosity and specificity in the Zum?1DHP inbred strain and Zum?1DHP outbred strain. The second Zum?1DHP subline becomes a new inbred strain guinea pig. It is es?sential that the subline with the characteristic property is screened from these sublines. The guinea pigs of black Zmu?2DHP inbred strain carrying microsatellite markers not present in the white strains, may carry optimal genes related with hair color properties.