Effect of tripterygium glycosides contained serum on pathological boneforming related inflammatory markers and miR-21 / 实用医学杂志
The Journal of Practical Medicine
; (24): 367-370, 2017.
Article
en Zh
| WPRIM
| ID: wpr-511588
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the effect of tripterygium glycosides (TG) contained serurn on the pathological boneforming related inflammatory markers and miR-21.Methods Previous isolated and cultured AS fibroblasts were stimulated using IL-1 of 1ng/ml for 24h,different concentrations of blank serum (5%,10%,and 15%) and TG contained serum (5%,10%,and 15%) were added for 48h.PGE-2,IL-17,IL-22,IL-23,CCL19 and CCL21 proteins were examined by Western blot.The osteogenesis marker BMP and microRNA-21 mRNAs were tested.Results 48 h after intervention,the expressions of inflammatory markers were obviously inhibited by TG contained serum;the boncforming related inflammatory markers,expressions of BMP-2 and miR-2t were all inhibited in a dose-dependent manner.Conclusions TG could inhibit the expressions of boneforming related inflammatory markers,BMP-2 and miR-21,thus providing theoretical basis to treat AS pathological boneforming.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
The Journal of Practical Medicine
Año:
2017
Tipo del documento:
Article