Screening and Optimization of the Extraction Technology of Total Flavonoids in Persimmon Leaves by Semi-bionic-enzyme Method / 中国药房
China Pharmacy
;
(12): 1813-1816, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-511671
ABSTRACT
OBJECTIVE:
To screen the method for extracting total flavonoids in persimmon leaves and optimize extraction tech-nology.METHODS:
Using extract quality and flavonoids content as indexes,the effects of extracting total flavonoids in persim-mon leaves by ethanol refluxing method,enzyme method(cellulase,β-glucanase and complex enzyme mixed by equal amounts of both),semi-bionic method and semi-bionic-enzyme method (the same enzymes) were compared. Using flavonoids content as in-dex,solid-liquid radio,reflux temperature,reflux time as factors,orthogonal test was designed to optimize the extraction technolo-gy conditions of flavonoids in persimmon leaves by semi-bionic-enzyme method,and the verification test was conducted. RE-SULTSThe extract quantity and flavonoids content by semi-bionic-enzyme method was the highest among the 4 extraction meth-ods,and the complex enzyme was the most suitable;the optimized extracting condition of semi-bionic-enzyme method were as fol-lows as solid-liquid radio of 114,reflux temperature of 50 ℃,reflux time of 2.0 h;extraction rates of flavonoids in 3 verification tests were 5.9%,5.8%,5.9%(RSD=0.98%,n=3).CONCLUSIONS:
The optimized semi-bionic-enzyme method is efficient and stable in extracting flavonoids in persimmon leaves.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
China Pharmacy
Año:
2017
Tipo del documento:
Artículo
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