Application of T-SPOT.TB in the Early Diagnosis of Tuberculosis / 现代检验医学杂志
Journal of Modern Laboratory Medicine
;
(4): 60-63, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-512710
ABSTRACT
Objective This study aims to explore the application value of tuberculosis T lymphocytes enzyme-linked immune SPOT test (T-SPOT.TB) on early diagnosis of tuberculosis.Methods The TB infection in 189 inpatients suspected tuberculosis in pneumology department of Shaanxi Provincial People's Hospital was detected with T-SPOT.TB,fluorescence RQPCR,tuberculosis (TB-Ab)protein chip and PPD methods.Results The sensitivity of four methods was 91.54% (119/130),73.85%(96/130),63.08%(82/130) and 57.69% (75/130) respectively and the specificity of those was 89.83% (53/59),86.44%(51/59),67.79%(40/59) and 66.10%(39/59),respectively.The sensitivity of T-SPOT.TB method was statistically higher than those of other three tests,respectively (P<0.05).The specificity of T-SPOT.TB was significantly higher than those of TB-AB and PPD (P<0.05),but there was no statistical difference between RQ-PCR and T-SPOT.TB (P>0.05).The positive predictive values of T-SPOT.TB,fluorescent quantitative PCR,TB-Ab and PPD assays were 95.2% (119/1250),92.3% (96/104),81.2% (82/101) and 78.9% (75/95) respectively while the negative predictive values of those were 82.8% (53/64),60% (51/85),45.5% (40/88) and 41.5% (39/94),respectively.The false-positive rates (misdiagnosis rate) of four assays were 10.2% (6/59),13.6% (8/59),32.2% (19/59) and 33.9% (20/59) respectively and the false-negative rates (rates of missed diagnosis) of those were 8.5% (11/130),26.2% (34/130),36.9% (48/130)and 42.3 % (55/130),respectively.The negative likelihood ratios of T SPOT.TB,fluorescent quantitative PCR,TB-Ab and PPD assays were 0.11,0.16,0.48 and 0.51 respectively,meanwhile the positive likelihood ratios of T-SPOT.TB,fluorescent quantitative PCR,TB-Ab andPPD assays were 9.0,5.4,2.0 and 1.7,respectively.What' s more,the diagnostic accordance rates of the four assays were 91.0% (172 189),77.8% (147 189).64.6% (122/189) and 60.3% (114/189),respectively.Conclusion T-SPOT.TB test is a more sensitive and specific method and of great significance to the early diagnosis of TB,which has more clinical value in different stages of tuberculosis diagnosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
Journal of Modern Laboratory Medicine
Año:
2017
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS