Change of Expression and Activity of Heme Oxygenase-1 in Rat Corpus Cavernosum during Low-flow Priapism / 대한남성과학회지
Korean Journal of Andrology
; : 81-86, 2004.
Article
en Ko
| WPRIM
| ID: wpr-51425
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: The inducible isoform of heme oxygenase(HO), HO-1, responds to hypoxia. HO-1 regulates vascular smooth muscle tone through carbon monoxide production. To investigate the possible role of HO-1 in low-flow priapism, we examined the expression and activity of HO-1 in artificially induced veno-occlusive priapism in rat. MATERIALS AND METHODS: Fourteen male Sprague Dawley rats were divided into 2 groups with 7 rats each. In the first group, low-flow priapism was induced using a vacuum-constriction device and a constriction rubber band; in the second group, low-flow priapism was induced using papaverine. We measured the expression level and activity of HO-1 in penile tissues after time periods of 0(control), 2, 3, 4, 8, 12, 24, and 48 hours. At the same time, the expression levels of i-NOS, e-NOS, and beta-actin(control) in penile tissues were also measured. RESULTS: In both groups, expression of HO-1 and HO-1 enzyme activities in penile tissue significantly increased in a time dependent fashion(p<0.01). However, there was no difference in the expression of i-NOS and e-NOS in both groups at any time period. CONCLUSIONS: HO-1 was induced over time in rats with artificially induced veno-occlusive priapism. Induction of HO-1 may play a protective role against hypoxic injury, but may also play an important role in the vicious cycle observed for low flow priapism. Increasing induction of HO-1 against hypoxic injury in a prolonged erectile state promotes sustained dilatation of corporal smooth muscle, and this may aggravate low-flow priapism.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Papaverina
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Priapismo
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Goma
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Monóxido de Carbono
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Ratas Sprague-Dawley
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Constricción
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Dilatación
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Hemo-Oxigenasa 1
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Hemo
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Hemo Oxigenasa (Desciclizante)
Límite:
Animals
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Humans
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Male
Idioma:
Ko
Revista:
Korean Journal of Andrology
Año:
2004
Tipo del documento:
Article