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Biocompatibility and quantitative analysis of oral bone implant materials in vivo using synchrotron radiation-based micro-computed-tomography:study protocol for a randomized controlled animal experiment / 中国组织工程研究
Chinese Journal of Tissue Engineering Research ; (53): 952-956, 2017.
Artículo en Chino | WPRIM | ID: wpr-514784
ABSTRACT

BACKGROUND:

At present, bone substitute filling is mainly used for bone defect repair. In order to understand the effect on bone defect repair, it is necessary to look into the microstructure changes of bone defects after bone substitute implantation. Synchrotron radiation-based micro-computed-tomography (SR-μCT) can be used to make high-resolution, high-resolution three-dimensional imaging without slicing and dyeing, and has high scientific and clinical value.

OBJECTIVE:

To explore the feasibility of SR-μCT in micron-level bone osseointegration examination in oral medicine.

METHODS:

This randomized controlled animal experiment was completed at the Fifth Central Hospital of Tianjin, Tianjin, China. A rabbit model of mandibular defect was made in 24 male New Zealand white rabbits. The model rats were randomly divided into four groups and received autologous bone, Bio-oss bone meal,β-tricalcium phosphate powder and no implantation (negative control group) in the defective area, respectively. Bone samples, including the defect area and the surrounding normal bone tissue, were taken at 2, 4, 8 weeks postoperatively for SR-μCT examination, fol owed by histopathological examination, in order to observe the repairing effects of different types of bone implant materials from different angles. The study protocol has been approved by the Ethics Committee of the Fifth Central Hespital of Tianjin in China. The study procedures were completed in accordance with the Guidance Suggestions for the Care and Use of Experimental Animals of China and the guidelines of the National Institutes of Health, USA. RESULTS AND

CONCLUSION:

In this study, SR-μCT could be used to observe the bone microstructure and osseointegration with no damage to samples to collect accurate quantitative data, including bone volume, number of bone trabeculae and bone mineral density. Therefore, SR-μCT can fully analyze the biocompatibility of bone implant material in vivo, give insight into the micron-level changes of different types of bone implant materials in the bone defect, thereby providing experimental evidence to improve bone defect healing.
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Ensayo Clínico Controlado / Guía de Práctica Clínica Idioma: Chino Revista: Chinese Journal of Tissue Engineering Research Año: 2017 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Ensayo Clínico Controlado / Guía de Práctica Clínica Idioma: Chino Revista: Chinese Journal of Tissue Engineering Research Año: 2017 Tipo del documento: Artículo