Effects of plasma non-specific immunosuppressive protein (PNIP) on adhesion of lymphocytes to aortic endothelial cells (EC) in rats / 中国病理生理杂志

ABSTRACT

Adhesion of lymphocytes to EC is one of the important steps of lympho. cyte migration into the tissues outside vessels; and the components of blood plasma may have some effect on the adhesion of lymphocytes. In our experiments, we observed that PNIP, normally present in blood and being able to inhibit the proliferation of lymphocytes stimulated by antigens or mitogens, could significantly inhibit the adhesion of the lymphocytes from blood, thymus and mesenteric lymph nodes (MLN) to the aortic EC, using the aortic EC monolayer preparation (Hautchen technique). Meanwhile, the indirect immunofluorescent test also showed us that PNIP might result in a remarkable decrease in percentage of SmIgG positive B lymphocytes in the total lymphocytes adhering to the aortic EC. These results imply that PNIP may suppress the adhesion of lymphocytes or other leukocytes to EC in the development of inflammation, atherosclerosis or thrombosis.