Effect of carbon monoxide on hypoxic proliferation of rat pulmonary arterial smooth muscle cell / 中国病理生理杂志

ABSTRACT

AIM:To explore the effect of carbon monoxide (CO) on hypoxic proliferation of rat pulmonary arterial smooth muscle cell(PASMC). METHODS:Rats were randomly divided into four groups, including (1)NNormoxia group (21% O 2); (2)HHypoxia group (1% O 2); (3)CO+HCO+hypoxia group (3% CO+1% O 2),tested by immunocytochemical analysis,[3H]-TdR incorporation and flow cytometry (FCM). RESULTS: (1)Flow cytometric DNA analysis revealed that hypoxia induced significantly enhancement of G 2/M phase and decreased G 0/G 1 phase of PASMC ( P