Determination of Propofol Concentration in Human Serum With RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To develop a RP-HPLC method for determining serum concentration of propofol METHODS:Using ODS C18 column as fixed phase,a mixture of methanol and water(75∶25) as mobile phase,excitation wavelength 270nm,emission wavelength 295nm RESULTS:The linear range was 0 0 375～8 0?g/ml,r=0 9 996 The within-day and between-day RSDs were less than 5%,the average recovery was 83 98% CONCLUSION:This is a good method to monitor propofol serum concentration