Determination of the Concentration of 5-Fluorouracil in Human Plasma With HPLC / 中国药房
China Pharmacy
;
(12)1991.
Artículo
en Chino
| WPRIM
| ID: wpr-519193
ABSTRACT
OBJECTIVE:
To establish a HPLC method for the determination of 5-fluorouracil in human plasmaMETHODS:
The plasma samples were extracted with ethyl acetate-isopropanol(85∶15,V/V)following precipitation of plasma proteins with ammonium sulfate The solvent was evaporated to dryness under a stream of nitrogen The dry extract was dissolved in mobile phase and then injected into the Diamonsil C18 column(150mm?4 6mm,5?m)and 0 01mol/L KH2 PO4(pH5 5) was taken as mobile phase The flow rate was 1 5ml/min,UV detection wavelength was 267nm The internal standard was 5-bromouracilRESULTS:
The minimal detectable drug concentration in plasma was 0 025?g/ml The calibration curve was linear over a range from 0 3?g/ml to 10?g/ml The intra-day RSD≤5 0%(n=5) and the inter-day RSD≤11 5%(n=5)CONCLUSION:
The method can be used for studying the pharmacokinetics and bioavailability of 5-fluorouracil
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
1991
Tipo del documento:
Artículo
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