Study on the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes of mouse / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1999.
Artículo
en Chino
| WPRIM
| ID: wpr-519663
ABSTRACT
AIM:
To study the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes (SCDC) of mouse.METHODS:
Embryonic stem cells of D 3 line (ES-D 3) were cultured on the MEF feeder layer with BRL conditioned medium, and fetal mouse heart cells(FMHC)were cultured in vitro. Then ES-D 3 cells were induced to differentiate into many kinds of cells. SCDC were harvested on day 12 after differentiation initiating and identified by electro-microscope and immunocytochemistry. SCDC and FMHC were prepared for the patch-clamp research. Sodium and calcium currents together were elicited and compared between SCDC and FMHC.RESULTS:
The current characteristics of sodium and calcium channels of SCDC were very similar to FMHC.CONCLUSION:
The functional expression of ion channels occurred during ES-D 3 cells differentiation and the electrophysiological characteristics of sodium and calcium channels of SCDC are very similar to FMHC.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
1999
Tipo del documento:
Artículo
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