Whole sequence amplification of an expressed sequence tag in immune cells of F344 rats with severe burns / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Artículo
en Chino
| WPRIM
| ID: wpr-520406
ABSTRACT
AIM:
To examine the changes in immune cells which might be involved in systemic immune dysfunction after severe burns, we detected the sequence of expressed sequence tag in lymphocytes and monocytes of F344 rats post-burn.METHODS:
Two pairs of primers were designed depending on the sequence of AI764697.1. SMART RACE technology was employed for amplification of whole sequence. Furthermore, the amplified sequence was testified by Northern blot.RESULTS:
A 592bp sequence, which was testified by Northern blot, was suceessfally obtained in our experiments. This product was accepted by GenBank and its accession number is AF244895.CONCLUSION:
A cDNA whole sequence in immune cells of F344 rats after severe burns was amplified successfully. More research work should be done to identify the resource, location in genome and function of this product.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
1986
Tipo del documento:
Artículo
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