The influence of peroxisome proliferator-activated receptors on transcription of plasminogen activator inhibitor-1 in endothelial cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Artículo
en Chino
| WPRIM
| ID: wpr-521050
ABSTRACT
AIM:
To investigate the expression of peroxisome proliferator-activated receptors (PPARs) in human endothelial cells, and their effects on plasminogen activator inhibitor-1 transcription.METHODS:
The expression of three types of PPARs in mRNA level were detected in human umbilical vein endothelial cells(HUVECs) by using RT-PCR. Cultured endothelial cells line-ECV304 were transfected with PAI-1 promoter controlling CAT reporter gene and co-transfected with varying doses (250, 500, 1 000 ng) of expression vectors PPAR? or PPAR?.The transcripton activity of PAI-1 promoter were detected with ELISA.RESULTS:
There were all three types of PPARs mRNA expression in HUVECs, while the expression of PPAR? was less than that of PPAR?( P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
2000
Tipo del documento:
Artículo
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