Rat mesenchymal stem cells differentiate into neuron-like cells induced by berberine in vitro / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Artículo
en Chino
| WPRIM
| ID: wpr-521948
ABSTRACT
AIM:
To investigate whether berberine can ind uce rat mesenchymal stem cells (MSCs) to differentiate into neuron -like cells in vitro.METHODS:
MSCs were separated from young rat femurs marrow and expanded in culture medium. MSCs were induced to di fferentiate by berberine. The morphological changes of MSCs were evaluated by li g ht microscope.Neuron-spcific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry.RESULTS:
Induced by be rberine for 8 hours,MSCs exhibited neurotype . The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express.CONCLUSION:
Berberine may induce adult rat MSCs to differe ntiate into neuron-like cells in vitro.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
1986
Tipo del documento:
Artículo
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