The c-Myc expression and apoptosis of mouse thymocytes treated with dexamethasone / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Artículo
en Chino
| WPRIM
| ID: wpr-522457
ABSTRACT
AIM:
To study c-Myc expression and its relationship with caspase-3 in a dexamethasone (DEX)- induced mouse thymocyte apoptosis model, and discuss the role of c-Myc in cell apoptosis.METHODS:
Mouse thymocyte apoptosis was induced by 1 ?mol/L DEX, the apoptotic and necrosis cells were measured by Annexin V-FITC/PI double staining flowcytometry at 30 min, 3 h, 6 h and 9 h . Electron microscopy observation was carried out at 6 h, and c-Myc and caspase-3 contents were tested by Western blot at 0, 30, 60, 180 min.RESULTS:
By 1?mol/L DEX treatment, the apoptosis rates of thymocytes at 30 min, 3 h, 6 h, 9 h were (5.70?0.46)%, (35.79?1.13)%, (50.61?2.15)% and (35.52?1.66)%,respectively; in control group, they were (5.97?0.25)%, (10.20?0.71)%, (12.10?0.66)% and (15.45?0.51)% (P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
1986
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS