Construction of human neuronal nitric oxide synthase expression system in Escherichia coli / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Artículo
en Chino
| WPRIM
| ID: wpr-522764
ABSTRACT
AIM:
To construct a high-level expression system of recombinant human neuronal nitric oxide synthase (hnNOS) full-length enzyme in Escherichia coli.METHODS:
The coding sequence of hnNOS full-length was firstly amplified by PCR, and then ligated into the expression vector pCWori+. The recombinant plasmid was transformed into Escherichia coli BL21 for high-level expression. After having been checked with Western blot, the enzyme was used for large-scale culture and purification. Finally, the property of the enzyme was determined by spectrophotometric method.RESULTS:
The constructed expression system could give a yielding of 3 mg/L initial culture.CONCLUSION:
The expression system constructed is fully sufficient to express the active human neuronal nitric oxide synthase.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Pathophysiology
Año:
1989
Tipo del documento:
Artículo
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