Determination of Chlorogenic Acid in Qingyan Granules by RP-HPLC / 中国药房
China Pharmacy
;
(12)1991.
Artículo
en Chino
| WPRIM
| ID: wpr-522832
ABSTRACT
OBJECTIVE:
To establish a RP-HPLC method for the determination of chlorogenic acid in Qingyan granules.METHODS:
Waters Breeze System was used with a Hypersil BDS C18 column(5?m,4.6mm?150mm).The mobile phase was composed of methanol and 0.4%(V/V)H3PO4 water solution(20∶80,V/V),flow rate1.0ml/min;wavelength of UV detector327nm,column temperature 20℃.RESULTS:
The calibration curve of chlorogenic acid was linear within the range of (174~1 392)ng(r=0.9 999).The average recovery was 100.10%,RSD=1.60%(n=5).CONCLUSION:
This method is simple,sensitive,as well as reliable,and is available to quality control.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
1991
Tipo del documento:
Artículo
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