Detection of Aconitine and Mesaconitine and Hypaconitine in blood by LC/MS/MS / 中国法医学杂志
Chinese Journal of Forensic Medicine
;
(6)2002.
Artículo
en Chino
| WPRIM
| ID: wpr-523542
ABSTRACT
Objective To establish a method used for determination of Aconitine and Hypaconitine and Mesaconitine in blood by LC/MS/MS.Methods Extraction of blood sample was conducted by use of 1% thirdchloroactic acid and acetonitrile liquid,and the electrospray fog ionic source,and positive ion MRM scan were employed.Result The linearity correlation coefficients(r)was≥0.992 2.The limit of quantification (LOQ S/N=5) was 2.0 ng/ml for Aconitine,0.5 ng/ml for Hypaconitine,and 0.5 ng/ml for Mesaconitine.The recovery of appended contrast in blank blood sample ranged from 91.25% to 103.1% with coefficient of variation (CV,n=6) less than 10.93%.Conclusion The method of LC/MS/MS is sensitive and reliable,and the sample handling is quick and simple,which was suitable for determination of the Aconitine and Hypaconitine and Mesaconitine in blood sample.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Chinese Journal of Forensic Medicine
Año:
2002
Tipo del documento:
Artículo
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