Relationship of Genomic Characteristics,Virulence of Sporothrix Sche nckii and Clinical Patterns of Sporotrichosis / 中华皮肤科杂志

ABSTRACT

Objective To explore the relationship of genomic characte ristics,virulence of S.schenckii and clinical patterns of sporotrichosis.Met hods Isolates of S.schenckii were collected from different clinical patterns of sporotrichosis.Genomic DNAs of S.schenckii isolates were amplified by rando mly amplified polymorphic DNA (RAPD).Suspensions of different isolates of S.sc henckii were inoculated into BALB/c mice in order to observe their pathogenicity.Histopathology of the skin and viscera of the mice was studied to determine th e numbers and distribution of spores.Results The electrophoretic profiles of PCR products differed in the isolates from different clinical patterns of sporo trichosis1 800 bp,850 bp,500 bp,and 180 bp in disseminated sporotrichosis;1 400 bp,800 bp,700bp,and 500 bp in the regional lymphangitic sporotrichosis,2 500 bp,1400 bp,1000 bp,and 700 bp in fixed cutaneous sporotrichosis.The earl ier onset of illness,wider distribution of lesions and higher mortality rate we re observed in the BALB/c mice inoculated with isolates from disseminated sporot richosis,followed by those from regional lymphangitic sporotrichosis and those from fixed cutaneous sporotrichosis.Histopathologically more spores were found in the mice inoculated by isolates from disseminated sporotrichosis,followed by those from regional lymphangitic sporotrichosis and those from fixed cutaneous sporotrichosis.Conclusions There is a strong correlation among genomic charac teristics,virulence of S.schenckii and clinical patterns of sporotrichosis.