Content Determination of the 3 Constituents in Jiangzhiyin by HPLC Simultaneous / 中国药房
China Pharmacy
;
(12)2005.
Artículo
en Chino
| WPRIM
| ID: wpr-525310
ABSTRACT
OBJECTIVE:
To establish a method for simultaneous determination of the effective constituents rhein,emodin and chrysophanol in jiangzhiyin by HPLC.METHODS:
SHIMADU VP-ODS column was used as analytical column,methanol-0.2%phosphoric acid(80∶20)as mobile phase,the flow rate was set at1ml/min,the detection wavelength at225nm,column temperature at room temperature.RESULTS:
The linear ranges of detected rhein,emodin and chrysophanol in jiangzhiyin were0.015~0.15?g(r=0.9996),0.014~0.14?g(r=0.9998)and0.0085~0.085?g(r=0.9993)respectively;The average recoveries were99.26%(RSD=2.00%),98.72%(RSD=1.80%)and99.76%(RSD=2.3%)respectively.CONCLUSION:
The determination results are stable and accurate,and the method is feasible and reliable,which can be used for the quality control of jiangzhiyin.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2005
Tipo del documento:
Artículo
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