Content Determination of Luteolin in Wild Chrysanthemum Flower by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a HPLC method for content determination of luteolin wild chrysanthemum flower.METHODS:The separation was performed on a Kromasil C18 column,the mobile phase was composed of tetrahydrofuran-methanol-1.5%acetic acid(27∶4∶69),the flow rate was 1.0 ml/min,the ultraviolet wavelength was 353nm,the column temperature was 30℃.RESULTS:The calibration curve showed good linearity in the range of 0.028?g～0.56?g(r=1.0 000),the average recovery was 99.9%(RSD=2.28%).CONCLUSION:The method is simple,convenient and accurate and can be used for the quality control of wild chrysanthemum flower.