Content Determination of Sophocarpidine in Fuyankang Concentrated Pill by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To determine the content of sophocarpidine in Fuyankang concentrated pill by HPLC.METHO_ DSThe loading agent of chromatographic column was amino bonded silica,the mobile phase was acetonitrile-dehydrated al?cohol-3%phosphoric acid(80∶10∶10),and the detection wavelength was220nm.RESULTS:There was a good linear rela?tionship between sample size and peak area score of sophocarpidine in the range of0.4064?g～2.0320?g,the average recovery rate was99.2%(RSD=1.6%).CONCLUSION:The established method is more accurate than TLC,and it can be used for the quality control of Fuyankang concentrated pill.