Functional changes of regulatory T cells in patients with Graves disease / 中国医师进修杂志

ABSTRACT

Objective To search the immune-mechanism of Graves disease,and get known the function of regulatory T cell in its occurrence.Methods Flow cytometry were used to detect the proportion of CD_4~+CD_(25)~+ T cells in 32 Graves disease patients(GD group) and 20 healthy volunteers(control group).MACS were used to isolate CD_4~+ CD_(25)~+ T cells.RT-PCR was used to detect the expression of FOXP3 of them,and ELISA to detect the level of IL-10.Results There was no significant change of proportion of CD_4~+CD_(25)~+ T cells between GD group and control group(P=0.804),while expression level of FOXP3 mRNA and secretion level of IL-10 in GD group were lower than those in control group(P