Trichostatin A, a Histone Deacetylase Inhibitor, Potentiated Cytotoxic Effect of Ionizing Radiation in Human Head and Neck Cancer Cell Lines / 대한방사선종양학회지
The Journal of the Korean Society for Therapeutic Radiology and Oncology
; : 138-144, 2004.
Article
en En
| WPRIM
| ID: wpr-52746
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: We have previously reported that human glioblastoma cells are sensitized to radiation-induced death after their exposure to trichostatin A (TSA), a histone deacetylase inhibitor (HDAC-I), prior to the irradiation. We aimed to measure the magnitude of the radiosensitizing effect of TSA in human head and neck cancer cell lines. MATERIALS AND MEHTODS: Human head and neck cancer cell lines, HN-3 and HN-9, were exposed to 0, 50, 100, and 200 nM TSA for 18 hr prior to irradiation. Then, the TSA-treated cells were irradiated with 0, 2, 4, 6, and 8 Gy, and cell survival was measured by clonogenic assay. RESULTS: Pre-irradiation exposure to TSA was found to radiosensitize HN-3 and HN-9 cell lines. In HN-9 cells, the fraction surviving after 2 Gy (SF2) was significantly reduced by treatment of TSA at concentration as low as 50 nM. However, a treatment with 200 nM TSA was required to significantly decrease SF2 in the HN-3 cell line. SER of pre-irradiation treatment with 200 nM TSA was 1.84 in HN-3 and 7.24 in HN-9, respectively. CONCLUSIONS: Our results clearly showed that human head and neck cancer cell lines can be sensitized to ionizing radiation by pre-irradiation inhibition of histone deacetylase (HDAC) using TSA, and that this potentiation might well be a general phenomenon.
Palabras clave
Texto completo:
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Índice:
WPRIM
Asunto principal:
Radiación Ionizante
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Fármacos Sensibilizantes a Radiaciones
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Histonas
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Línea Celular
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Supervivencia Celular
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Glioblastoma
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Inhibidores de Histona Desacetilasas
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Cabeza
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Neoplasias de Cabeza y Cuello
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Histona Desacetilasas
Límite:
Humans
Idioma:
En
Revista:
The Journal of the Korean Society for Therapeutic Radiology and Oncology
Año:
2004
Tipo del documento:
Article