Protective effects of transection of cervical sympathetic trunk on gastric mucosal lesions induced by stress in rats / 中华麻醉学杂志

ABSTRACT

Objective To investigate the protective effects of transection of the cervical sympathetic trunk (TCST) on the gastric mucosal lesions in rats fastened to a board and immersed vertically in water, up to the level of xiphoid with the animals' heads up water for 6 hours. Methods Thirty male SD rats weighing 200-250 g were randomly divided into 3 groups ( n = 10 each) group A sham operation; group B sham operation + water immersion and group C TCST + water immersion. The animals were anesthetized with intraperitoneal 2.5% pentobarbital 40 mg?kg-1. Cervical sympathetic trunk was exposed at right common carotid artery bifurcation and cut. The gastric mucosal blood flow (GMBF) was measured with Doppler blood flow monitor after 6 h water immersion. Blood samples were taken from abdominal aorta for determination of plasma concentration of ET-1 and serum concentration of NO. Gastric mucosal ulcer index was determined according to Guth criteria.Results There was gastric mucosa bleeding and erosion in group B and C and the degree of injury was severer in group B than in group C. Plasma concentration of ET-1 and serum concentration of NO were significantly higher in group B than in group C and A.Conclusion TCST has protective effect on gastric mucosal blood flow in rats under stress by reducing blood ET and NO concentrations.